Efficient method to create integration-free, virus-free,MycandLin28-free human induced pluripotent stem cells from adherent cells
نویسندگان
چکیده
منابع مشابه
Efficient method to create integration-free, virus-free, Myc and Lin28-free human induced pluripotent stem cells from adherent cells
AIM Nonviral induced pluripotent stem cell (IPSC) reprogramming is not efficient without the oncogenes, Myc and Lin28. We describe a robust Myc and Lin28-free IPSC reprogramming approach using reprogramming molecules. METHODS IPSC colony formation was compared in the presence and absence of Myc and Lin28 by the mixture of reprogramming molecules and episomal vectors. RESULTS While more colo...
متن کاملAn efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells.
The generation of induced pluripotent stem cells (iPSCs) provides the opportunity to use patient-specific somatic cells, which are a valuable source for disease modeling and drug discovery. To promote research involving these cells, it is important to make iPSCs from easily accessible and less invasive tissues, like blood. We have recently reported the efficient generation of human iPSCs from a...
متن کاملMelanocytes Derived from Transgene-Free Human Induced Pluripotent Stem Cells
Odontostomatologiche, Ortodontiche e Chirurgiche, di Oftalmologia, di Patologia della Testa e del Collo, del Cavo Orale e della Comunicazione Audio Verbale e Scienze Ortopediche, Tramautologiche, Riabilitative e Plastico-Ricostruttive, Seconda Università di Napoli, Naples, Italy and Dipartimento di Chimica Farmaceutica e Tossicologica, Facoltà di Farmacia, Università degli Studi di Napoli ‘Fede...
متن کاملHighly Efficient Neural Conversion of Human Pluripotent Stem Cells in Adherent and Animal‐Free Conditions
Neural differentiation of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) can produce a valuable and robust source of human neural cell subtypes, holding great promise for the study of neurogenesis and development, and for treating neurological diseases. However, current hESCs and hiPSCs neural differentiation protocols require either animal factors or embryoid bo...
متن کاملIntegration-free Methods for Generating Induced Pluripotent Stem Cells
Induced pluripotent stem (iPS) cells can be generated from mouse or human fibroblasts by exogenous expression of four factors, Oct4, Sox2, Klf4 and c-Myc, and hold great potential for transplantation therapies and regenerative medicine. However, use of retroviral vectors during iPS cell generation has limited the technique's clinical application due to the potential risks resulting from genome ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Future Science OA
سال: 2017
ISSN: 2056-5623
DOI: 10.4155/fsoa-2017-0028